![]() ![]() 45 min), thus avoiding excess protein modification and degradation after extended-period incubation in TCA/acetone or acetone. Compared to classical TCA/acetone precipitation and simple acetone precipitation, this protocol generates comparable yields, spot numbers, and proteome profiling, but takes less time (ca. Proteins of cells or tissues were extracted using SDS-containing buffer, precipitated with equal volume of 20% TCA/acetone, and washed with acetone. We have described a modified method of TCA/acetone precipitation of plant proteins for proteomic analysis. In addition, by prolonged incubation in TCA/acetone, the precipitated proteins are more difficult to re-dissolve. However, the TCA/acetone precipitation method relies on the complete pulverization and repeated rinsing of tissue powder to remove the interfering substances, which is laborious and time-consuming. TCA/acetone precipitation and phenol extraction, a common method of protein extraction, is thought to minimize protein degradation and activity of proteases as well as reduce contaminants like salts and polyphenols. ![]() A number of protein extraction methods have been applied to various biological samples. Protein extracts obtained from cells or tissues often require removal of interfering substances for the preparation of high-quality protein samples in proteomic analysis. ![]()
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